【公開日：2024.07.25】【最終更新日：2024.06.12】
課題データ / Project Data
課題番号 / Project Issue Number
23CT0085
利用課題名 / Title
酸化鉄ナノ粒子を用いた抗トキソプラズマ薬の開発
利用した実施機関 / Support Institute
公立千歳科学技術大学 / Chitose IST
機関外・機関内の利用 / External or Internal Use
外部利用/External Use
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）物質・材料合成プロセス/Molecule & Material Synthesis（副 / Sub）計測・分析/Advanced Characterization
【重要技術領域 / Important Technology Area】（主 / Main）次世代バイオマテリアル/Next-generation biomaterials（副 / Sub）マルチマテリアル化技術・次世代高分子マテリアル/Multi-material technologies / Next-generation high-molecular materials
キーワード / Keywords
電子顕微鏡/ Electronic microscope,バイオアダプティブ材料/ Bioadaptive materials,抗菌・抗ウイルス材料/ Antibacterial/antiviral materials
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
加藤 健太郎
所属名 / Affiliation
東北大学大学院農学研究科
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
Oluyomi Stephen Adeyemi
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
利用形態 / Support Type
（主 / Main）技術代行/Technology Substitution（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
CT-002：フーリエ変換赤外分光光度計（FTIR)/赤外顕微鏡
CT-012：透過型電子顕微鏡（TEM）
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
We screened nanoparticles (NPs) of titanium dioxide and molybdenum for their potential to inhibit the growth of T. gondii in vitro. Previously, we showed that amino acid modification of NPs enhances their selective anti-parasite toxicity. Therefore, to enhance the selective anti-parasitic action of TiO2 , We modified the NP surface using alanine, aspartate, arginine, cysteine, glutamate, tryptophan, tyrosine, and bovine serum albumin. Of the eight bio-modified TiO2 , tryptophan-TiO2 showed the most promising anti- T. gondii specificity and improved host biocompatibility.
実験 / Experimental
For the in vitro model of experimental toxoplasmosis, we used the RH 2F strain of T. gondii. T. gondii RH strain 2F is a luciferase-reporting parasite; therefore, determination of parasite viability was achieved by using a luminescence-based assay of the β-galactosidase (β-gal) activity of the parasite. Initially, we screened the NPs of TiO2 and Mo for in vitro growth inhibition of T. gondii at various concentrations (between 0.0 and 1000 µg/mL). In addition, we performed a host cytotoxicity assessment at the same concentrations. Based on the initial results, we selected TiO2 NPs for subsequent bio-modification experiments.
結果と考察 / Results and Discussion
We confirmed successful bio-modification of the TiO2 NPs by using UV/Vis absorption spectroscopy. Since the surface plasmon resonance (SPR) absorption bands are distinctive characteristics for stable inorganic NPs, UV/Vis absorption spectroscopy confirmed the effective modification of the TiO2 NPs. The SPR absorption bands for the NPs (modified and naked) were between 190 and 339 nm. TEM images showed that the modified nanoparticles were of different shapes. Furthermore, the bio-modification appeared not to have appreciably affected the diameter of the TiO2 NPs, as shown in the TEM micrographs. In the TEM analyses, the size of the particles remained similar regardless of the presence or type of amino acid.
図・表・数式 / Figures, Tables and Equations
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
-
Oluyomi Stephen Adeyemi, L-tryptophan-titanium oxide nanoparticles showed selective anti-Toxoplasma gondii activity and improved host biocompatibility, Biomedicine & Pharmacotherapy, 162, 114597(2023).
DOI: 10.1016/j.biopha.2023.114597
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件