【公開日：2024.07.25】【最終更新日：2024.05.21】
課題データ / Project Data
課題番号 / Project Issue Number
23UT0144
利用課題名 / Title
エクソソームと微生物の表面分析
利用した実施機関 / Support Institute
東京大学
機関外・機関内の利用 / External or Internal Use
内部利用（ARIM事業参画者以外）/Internal Use (by non ARIM members)
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）計測・分析/Advanced Characterization（副 / Sub）-
【重要技術領域 / Important Technology Area】（主 / Main）次世代バイオマテリアル/Next-generation biomaterials（副 / Sub）次世代ナノスケールマテリアル/Next-generation nanoscale materials
キーワード / Keywords
電子顕微鏡/ Electronic microscope,バイオアダプティブ材料/ Bioadaptive materials,ナノ粒子/ Nanoparticles
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
Wang Chenyu
所属名 / Affiliation
東京大学 大学院工学系研究科
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
寺西亮佑
利用形態 / Support Type
（主 / Main）機器利用/Equipment Utilization（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
Probiotics are beneficial microorganisms, typically bacteria or yeast, that offer various health advantages when consumed in adequate amounts. They are known for promoting a balanced gut microbiome and supporting digestive and immune system wellness. Delivering live probiotics orally for treating gut disorders holds promise, but the harsh gastrointestinal (GI) conditions hinder their effectiveness. To overcome these challenges, we proposed the use of biomaterial-based nanocoatings to shield probiotics. These coatings not only protect probiotics from antibiotics and the hostile GI environment but also aid in their retention within the GI tract. Specifically, our study focuses on a novel metal-phenolic network-based single-cell nanocoating, composed of Fe3+ and quercetin, which effectively protects probiotics from antibiotic action. The morphology of probiotics before and after surface functionalization was characterized by TEM.
実験 / Experimental
Initially, we prepared Fe-Q capsules through a self-assembly process to investigate the optimal conditions for Fe3+ ion coordination with quercetin (Q). The morphology of these Fe-Q capsules was characterized using Transmission Electron Microscopy (TEM). Subsequently, E. coli was utilized as a probiotic template. Comparative studies were conducted on bare E. coli and Fe-Q coated E. coli, with both types being characterized through TEM (JEM-1400).
結果と考察 / Results and Discussion
The Fe-Q capsules demonstrated round shapes, thin films, and uniform sizing, with diameters averaging around 3000 nm. However, a notable difference was observed when comparing the surface roughness of the capsules. The 2-layer coated Q-Fe capsules (Figure a) had a relatively smooth surface, whereas the 5-layer coated capsules (Figure b) exhibited a rougher surface. This suggests that the surface roughness increases with the number of coating layers. The morphology of E. coli with uranyl acetate staining is shown in Figure c. This image reveals a smooth surface and distinct, individual particles. However, the TEM image in Figure d, taken after the MPN coating process, shows the formation of an ultrathin layer on the surface of the E. coli. Furthermore, it is observed that several E. coli cells tend to aggregate following the MPN coating.
図・表・数式 / Figures, Tables and Equations
Figure. Morphology of Fe-Q MPN capsules with 2 layers (a) and 5 layers (b). TEM images of bare E. coli with uranyl acetate staining (c) and MPN coated E. coli (d).
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件