【公開日：2023.07.31】【最終更新日：2023.05.31】
課題データ / Project Data
課題番号 / Project Issue Number
22MS1007
利用課題名 / Title
Interaction of polymers with lipids
利用した実施機関 / Support Institute
自然科学研究機構 分子科学研究所 / IMS
機関外・機関内の利用 / External or Internal Use
外部利用/External Use
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）物質・材料合成プロセス/Molecule & Material Synthesis（副 / Sub）-
【重要技術領域 / Important Technology Area】（主 / Main）次世代バイオマテリアル/Next-generation biomaterials（副 / Sub）-
キーワード / Keywords
Cationic Polymers, Membrane Interactions, Metal coordination
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
Robin Rajan
所属名 / Affiliation
北陸先端科学技術大学院大学先端科学技術研究科
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
利用形態 / Support Type
（主 / Main）機器利用/Equipment Utilization（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
With the known limitations of the pre-existing drugs the understanding of mechanism of the newly formed drugs is required. To achieve that purpose, we synthesized different types of cationic polymers and studied their interaction with the model membrane systems. In all a series of Zn-bound and non-bound polymers were synthesized and their interaction behavior was studied with these membrane systems.
実験 / Experimental
Isothermal titration calorimetry were performed by using MicroCal PEAQ-ITC (Malvern) using MicroCal PEAQ-ITC Control Software. The titrant syringe was filled with 40 µL of different types of polymers and the sample cell was filled with 200 µL of DOPC model membrane. The reference cell was filled with deionized water.
結果と考察 / Results and Discussion
Cancer is one of the leading causes of death worldwide. With the known limitations of small-molecule drugs and conventional drug delivery systems, the potential use of polymeric molecules as anticancer agents could be a game-changer in the field of polymer-based biopharmaceuticals. The mechanistic investigation of the interaction between the cationic homopolymers and the copolymers with the cancer cell membrane is required for better understanding. Therefore, understanding the membrane leakage and the interaction of the polymers are fundamentally and the practically very important. On the same side we should understand the complexities of the cell surface and the need to keep the cells viable makes it difficult to gain the fundamental understanding. For this purpose, the reduced physical systems based on the model membranes are appealing. Therefore, to fulfil our objective, to study the interactions between the polymers and the membrane we chose DOPC as the model membrane and studied the interaction with the model membrane via isothermal titration calorimetry. The interaction study of the PLL-NA-DDSA based polymers with Zn and without Zn was carried out on the DOPC as model membrane systems (Figure 1). Although a clear change in the in the enthalpy of the Zn bound and without Zn polymer was observed but a in depth analysis of the system is required.
図・表・数式 / Figures, Tables and Equations
Figure 1: Interaction of the PLL-NA-DDSA polymers and the PLL-NA-DDSA-Zn polymers with the DOPC as model membranes.
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件