【公開日：2024.07.25】【最終更新日：2024.05.14】
課題データ / Project Data
課題番号 / Project Issue Number
23NM5212
利用課題名 / Title
生化学作用を示す電極等の材料探索と評価
利用した実施機関 / Support Institute
物質・材料研究機構 / NIMS
機関外・機関内の利用 / External or Internal Use
内部利用（ARIM事業参画者以外）/Internal Use (by non ARIM members)
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）物質・材料合成プロセス/Molecule & Material Synthesis（副 / Sub）加工・デバイスプロセス/Nanofabrication
【重要技術領域 / Important Technology Area】（主 / Main）マテリアルの高度循環のための技術/Advanced materials recycling technologies（副 / Sub）量子・電子制御により革新的な機能を発現するマテリアル/Materials using quantum and electronic control to perform innovative functions
キーワード / Keywords
スパッタリング/ Sputtering,質量分析/ Mass spectrometry,赤外・可視・紫外分光/ Infrared/visible/ultraviolet spectroscopy
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
岡本 章玄
所属名 / Affiliation
物質・材料研究機構
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
HO Chia-Lun,徳納吉秀,四方 亜弓
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
服部晋也
利用形態 / Support Type
（主 / Main）機器利用/Equipment Utilization（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
NM-011：フーリエ変換赤外分光光度計
NM-013：ゼータ電位計
NM-018：HPLC(質量分析計付き)
NM-002：LC-MS（Q-Exactive Plus）
NM-641：スパッタ装置 [CFS-4EP-LL #2]
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
The measurements of NM-011 and NM-013 were for the characterization of magnetic nanomaterial applied to the enrichment of electrogenic bacteria, and the measurement of NM-018 was for the characterization of metabolites in the supernatant from bacterial solution.
実験 / Experimental
Magnetic nanomaterials with different types of linkers such as lysine or folic acid were covered with osmium tetroxide. NM-011 and NM-013 could check if the linker and osmium tetroxide were successfully covered on the material.Bacteria with a 5-day incubation produced different types of metabolites. With the filtration process removing the big particles such as bacterial cells and precipitates, the supernatant can be further applied to NM-018 for measuring the potential chemicals in the supernatant that release from cells.
結果と考察 / Results and Discussion
From the results of NM-011, we can observe the -OH and -NH peaks' appearance with the fabrication of linkers. Different linkers presented different spectra. With the addition of osmium tetroxide, the intensity of the peak near 2900 cm-1 increased, which is consistent with the spectrum of osmium tetroxide only (Fig 1). From the results of NM-013, we can observe the zeta potential difference in the different steps of the fabrication. The zeta potential of bare magnetic nanomaterial showed negative. With the linkers, the zeta potential of nanomaterial changed to positive. With osmium tetroxide, the zeta potential of nanomaterial changed to negative again (Fig 2). Therefore, based on the two measurements, we can confirm the success of the fabrication process.From the results of NM-018, we cannot observe any signals during the measurement. The possible reason may be that the C-18 column cannot properly separate the small molecules or maybe that most of the metabolites were lost during the filtration. Therefore, for future measurements, we are going to use the column that is specific for separating small molecules with the guide column to directly measure the samples without filtration.
図・表・数式 / Figures, Tables and Equations
Fig. 1 FT-IR spectra of magnetic nanomaterial
Fig. 2 Zeta potential of magnetic nanomaterial
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
DOI（論文・プロシーディング）：10.1016/j.cej.2023.142936
C. L. Ho, M. Y. Emran, S. Ihara, W. Huang, S. Wakai, W. P. Li, A. Okamoto “Osmium-grafted Magnetic Nanobeads Improve Microbial Current Generation via Culture-free and Quick Enrichment of Electrogenic Bacteria”, Chemical Engineering Journal,466,142936（2023).
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件