【公開日：2024.07.25】【最終更新日：2024.03.14】
課題データ / Project Data
課題番号 / Project Issue Number
23AT0332
利用課題名 / Title
Raman spectra of lyophilized urease powder
利用した実施機関 / Support Institute
産業技術総合研究所 / AIST
機関外・機関内の利用 / External or Internal Use
内部利用（ARIM事業参画者以外）/Internal Use (by non ARIM members)
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）計測・分析/Advanced Characterization（副 / Sub）-
【重要技術領域 / Important Technology Area】（主 / Main）次世代バイオマテリアル/Next-generation biomaterials（副 / Sub）-
キーワード / Keywords
生体由来素材/ Bio-based materials; ラマン分光/ Raman spectroscopy,バイオアダプティブ材料/ Bioadaptive materials,赤外・可視・紫外分光/ Infrared/visible/ultraviolet spectroscopy
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
Serien Daniela
所属名 / Affiliation
産業技術総合研究所
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
赤松 雅洋
利用形態 / Support Type
（主 / Main）機器利用/Equipment Utilization（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
We want to acquire Raman spectra for lyophilized urease powder to evaluate any changes before and after laser processing.
実験 / Experimental
We used the Raman microscope and focused directly onto the dry urease powder which was carefully mounted on a cover glass with double sided tape. With 532 nm 10mW laser, a 20× magnification, and a 50-micron-wide circular pinhole, we collected n=20 for 5 seconds each. The measurement was repeated on several powder pieces (Fig. 1a, b) and on several days.
結果と考察 / Results and Discussion
The resulting spectrum seems of good quality. However, in the fingerprint region (500-1800cm-1), peaks for alpha-helix are entirely absent. While we could not yet find explicit literature discussion of urease Raman data, urease contains alpha-helices and the peak's absence indicates some kind of error. Lyophilized bovine serum albumin for example has alpha-helices conserved even in powder form. So, this result is unexpected. Library search after baseline processing also indicates some less than 50% likeness with mannitol and cellulose acetate (Fig. 2). Possibly, erroneously the double sided tape contaminated the sample even though we carefully measured pieces on, near and further separated from the tape.
図・表・数式 / Figures, Tables and Equations
Fig.1a Powder on tape, 20x view, as measured.
Fig.1b Powder afar from tape
Fig.2 library search. Red spectrum is my result. Closest hits under 50%.
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件