【公開日：2024.07.25】【最終更新日：2024.05.16】
課題データ / Project Data
課題番号 / Project Issue Number
23UT1126
利用課題名 / Title
Single cell electrochemical aptasensors chip based on DEP trapping
利用した実施機関 / Support Institute
東京大学 / Tokyo Univ.
機関外・機関内の利用 / External or Internal Use
内部利用（ARIM事業参画者以外）/Internal Use (by non ARIM members)
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）加工・デバイスプロセス/Nanofabrication（副 / Sub）計測・分析/Advanced Characterization
【重要技術領域 / Important Technology Area】（主 / Main）次世代バイオマテリアル/Next-generation biomaterials（副 / Sub）-
キーワード / Keywords
DEP、dielectrophoresis、誘電永動、aptasensors、バイオセンサ,スパッタリング/ Sputtering,リソグラフィ/ Lithography,光リソグラフィ/ Photolithgraphy,電子線リソグラフィ/ EB lithography,膜加工・エッチング/ Film processing/etching,電子顕微鏡/ Electronic microscope,光学顕微鏡/ Optical microscope,バイオセンサ/ Biosensor
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
金 秀炫
所属名 / Affiliation
東京大学生産技術研究所
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
利用形態 / Support Type
（主 / Main）機器利用/Equipment Utilization（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
UT-505：レーザー直接描画装置 DWL66+2018
UT-503：超高速大面積電子線描画装置
UT-604：高速シリコン深掘りエッチング装置
UT-711：LL式高密度汎用スパッタリング装置(2019)
UT-506：枚葉式ZEP520自動現像装置
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
Micro/Nano patterns are fabricated on silicon/silicon oxide substrate by using laser writing or e-beam lithography, as well as DRIE, gold thin films are deposited by using sputtering machine.
実験 / Experimental
Laser lithography, E-beam lithography and metallization processes were used here. The details of the process are shown below.i. The substrate is cleaned by sonication in a bath of acetone and ethanol for 5min, respectively, then dried with blowing nitrogen at room temperature.ii. ZEP (HSQ) and JSR resists are used for E-beam and Laser writing, respectively.iii. E-beam exposure or Laser exposureiv. After e-beam or laser writing, the sample is put in the right developer for appropriate time and followed with rinsing under flowing DI water and dried with nitrogen.v. The gold thin films are deposited by using Sputter CFS-4EP-LL i-Miller.
結果と考察 / Results and Discussion
Figure 1(a) shows the optical microscope image of the gold electrodes pattern. Figure 1(b) presents the photo of the silicon chip with through holes fabricated by DRIE. Figure1(c) shows the scanning electron microscope (SEM) image of HSQ nanopillars.
図・表・数式 / Figures, Tables and Equations
Figure 1(a) Optical microscope image of the gold electrodes sample on Si substrate, (b) image of the Si chip with desired dimension of through holes, (c) SEM image to show HSQ nanopillars (diameter: 200 nm, pitch: 500 nm).
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
-
Shuo Li, Single-Cell Electrochemical Aptasensor Array, ACS Sensors, 8, 2921-2926(2023).
DOI: https://doi.org/10.1021/acssensors.3c00570
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件