【公開日：2023.08.01】【最終更新日：2023.05.22】
課題データ / Project Data
課題番号 / Project Issue Number
22KT1443
利用課題名 / Title
in vitroで臓器機能と腎毒性にアクセスする近位尿細管オンチップの実現
利用した実施機関 / Support Institute
京都大学 / Kyoto Univ.
機関外・機関内の利用 / External or Internal Use
内部利用（ARIM事業参画者以外）/Internal Use (by non ARIM members)
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）加工・デバイスプロセス/Nanofabrication（副 / Sub）-
【重要技術領域 / Important Technology Area】（主 / Main）マルチマテリアル化技術・次世代高分子マテリアル/Multi-material technologies / Next-generation high-molecular materials（副 / Sub）-
キーワード / Keywords
生体模倣,PDMS,尿細管,リソグラフィ/Lithography,異種材料接着・接合技術/ Dissimilar material adhesion/bonding technology
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
B.Sadeghian Ramin
所属名 / Affiliation
京都大学 大学院工学研究科
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
馬成,川上暎彦,久保亜樹,森分真由美
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
河野恵子
利用形態 / Support Type
（主 / Main）機器利用/Equipment Utilization（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
The renal proximal tubule is responsible for reabsorption of water, glucose, amino acids, and other molecules from the glomerular filtrate which are not meant for excretion. This project involves implementing multiple stages to model the organ in vitro using a microphysiological system. The reabsorption performance of a variety of on chip proximal tubule tissue configurations is currently under investigation. Here we report on our recent advances on the fabrication of the chip.
実験 / Experimental
PDMS-based chips were fabricated as outlined in the last fiscal report. Briefly, two identical PDMS slabs cast on Si-SU8 molds, bearing ‘S’ shaped microchannels, were bonded together with a PET membrane in between. Previously, we used a thin PDMS layer spun coated on one of the slabs and cured at room temperature to attach the slabs. This method has a downside. Uncured PDMS diffuses into the channel and reduces the apparent area of the membrane. To resolve this, the membrane and the bottom slab with microchannel side up were treated under O2 plasma for 1 min to render the surfaces hydrophilic. The membrane was then placed on the bottom slab. Next, the top slab along with the membrane-bearing bottom slab were exposed to O2 plasma for 1 min. The slabs were immediately brought together and bond under microscope without using an adhesion layer.
結果と考察 / Results and Discussion
Figs. 1a and 1b show photographs of proximal tubule cell-laden membranes formed on devices fabricated using PDMS- and plasma-based bonding methods, respectively. It is clear than the latter offers a wider cell coverage.
図・表・数式 / Figures, Tables and Equations
Fig. 1 Optical images of the (a) PDMS-based and (b) O2 plasma-based bonded cell-laden membranes.
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件