【公開日：2023.07.28】【最終更新日：2023.05.29】
課題データ / Project Data
課題番号 / Project Issue Number
22AT0074
利用課題名 / Title
Direct write particles
利用した実施機関 / Support Institute
産業技術総合研究所 / AIST
機関外・機関内の利用 / External or Internal Use
外部利用/External Use
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）加工・デバイスプロセス/Nanofabrication（副 / Sub）-
【重要技術領域 / Important Technology Area】（主 / Main）その他/Others（副 / Sub）次世代バイオマテリアル/Next-generation biomaterials
キーワード / Keywords
microfluidic devices, soft silicone elastomer, polydimethylsiloxane (PDMS), bacteria
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
Utada Andrew
所属名 / Affiliation
筑波大学
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
利用形態 / Support Type
（主 / Main）機器利用/Equipment Utilization（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
I have utilized the clean room facilities at AIST to fabricate molds for microfluidic devices. I used the Karl Suss mask aligner for photolithography to fabricate one- and two-layer masters. After fabricating the master, I replicated the features into the soft silicone elastomer called polydimethylsiloxane (PDMS). I subsequently bond the PDMS device to glass to form a completed device. I am able to trap bacteria in chambers of ~O(1 micron). We are still gathering data to understand how the bacteria grow in these confined environments.
実験 / Experimental
• 【NPF008】スピンコーター（フォト）
• 【NPF009】コンタクトマスクアライナー[MJB4]
• 【NPF014】有機ドラフトチャンバー_1
This year I have used the chemical bench, spin coaters, hot plates, and Karl Suss mask aligner. To do photolithography using photoresist SU-8, I first spin the resist on the wafers, then bake, and then expose using the Karl-Suss contact mask aligner.
結果と考察 / Results and Discussion
It is known that bacteria can grow in PDMS devices, which are useful for confining and culturing bacteria. The material is transparent and can be bonded to glass. Moreover, holes can be punched and tubes incorporated, which makes PDMS microfluidic devices very convenient for culturing and observing bacterial growth and facilitating analysis.
Since bacteria are usually approximately 1um in diameter, in order to visualize the cells, we must keep them confined to a monolayer. In order to do this, we need to make a “two-layer” device (see Fig. 1). When we grow bacteria in these chambers they remain as a monolayer enabling us to study their growth and behavior.
図・表・数式 / Figures, Tables and Equations
Fig 1（左）. Confocal images of our two-dimensional device alongside a color coded three dimensional image of the microfluidic device.
Fig 1（右）. Confocal images of our two-dimensional device alongside a color coded three dimensional image of the microfluidic device.
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件