【公開日：2024.07.25】【最終更新日：2024.07.05】
課題データ / Project Data
課題番号 / Project Issue Number
23SH0030
利用課題名 / Title
The mechanism of the rapid closure of the Venus Flytrap
利用した実施機関 / Support Institute
信州大学 / Shinshu Univ.
機関外・機関内の利用 / External or Internal Use
外部利用/External Use
技術領域 / Technology Area
【横断技術領域 / Cross-Technology Area】（主 / Main）計測・分析/Advanced Characterization（副 / Sub）-
【重要技術領域 / Important Technology Area】（主 / Main）次世代バイオマテリアル/Next-generation biomaterials（副 / Sub）-
キーワード / Keywords
バイオアダプティブ材料/ Bioadaptive materials,X線回折/ X-ray diffraction
利用者と利用形態 / User and Support Type
利用者名（課題申請者）/ User Name (Project Applicant)
ZENG XIANGLI
所属名 / Affiliation
大阪大学工学研究科
共同利用者氏名 / Names of Collaborators in Other Institutes Than Hub and Spoke Institutes
ARIM実施機関支援担当者 / Names of Collaborators in The Hub and Spoke Institutes
利用形態 / Support Type
（主 / Main）機器利用/Equipment Utilization（副 / Sub）-
利用した主な設備 / Equipment Used in This Project
報告書データ / Report
概要（目的・用途・実施内容）/ Abstract (Aim, Use Applications and Contents)
A Venus flytrap is a thigmotactic plant and able to predate insects under mechanical stimulation. To investigate the predation in detail, a fluorescent probe was used. However, in the case of using the confocal imaging, it is hard to obtain a 3D model both at a tissue and cell level. On the other hand, it is possible to obtain the 3D cell model of the Venus flytrap with a computed tomography. In this study, we aim to observe the tissue and cell within the Venus flytrap using 3D X-ray microscope (ZEISS Xradia 620 Versa) and obtain information of the mechanical system for the predation of the Venus flytrap.
実験 / Experimental
Two types of the Venus flytrap in a close and open state were measured with the X-ray microscope. Transmission and cross-sectional images of the Venus flytraps were obtained; we observed a leaf blade shown in Fig. 1.
結果と考察 / Results and Discussion
In Figs. 2 and 3, some trigger hairs were observed within the Venus flytrap regardless of the close and open state. Although there are six trigger hairs (thorns within leaf blades) actually, they were not clearly observed. In Figs. 3(C) and (D), tissues were observed; this tissue with the size of 70‒80 µm is thought to be a vascular bundle. In this study, we were able to observe internal structures of the Venus flytrap. However, it is difficult to elucidate the mechanical system for the predation on the basis of only information obtained with the X-ray microscope.
図・表・数式 / Figures, Tables and Equations
Fig. 1. A picture of the measured Venus flytrap. A red circle shows a leaf blade.
Fig. 2. Transmission photographs taken from (A) the front and (B) the side of the Venus flytrap in a close state. In the blue rectangle, multiple trigger hairs are observed.
Fig. 3. Transmission photographs taken from (A) the front and (B) the side of the Venus flytrap in an open state. In the blue rectangle, multiple trigger hairs are observed. Transmission photographs (C) and (D) are magnifications of (A) and (B), respectively.
その他・特記事項（参考文献・謝辞等） / Remarks(References and Acknowledgements)
成果発表・成果利用 / Publication and Patents
論文・プロシーディング（DOIのあるもの） / DOI (Publication and Proceedings)
口頭発表、ポスター発表および、その他の論文 / Oral Presentations etc.
特許 / Patents
特許出願件数 / Number of Patent Applications：0件
特許登録件数 / Number of Registered Patents：0件